FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A005.C80FNANXX.s_8.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A005.C80FNANXX.s_8.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29220820
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA1517010.5191538088253512No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA577190.19752696878458578No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC516580.1767849088423939No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC408970.139958426902462No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA398050.13622136545107222No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA360880.12350098320307232No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT328020.11225557667443967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT501400.039.1899721
CAAATAT525450.036.014623
ACAAATA569450.032.8767172
GCGCACG95650.032.041646
TAATTAT618650.029.7977039
AATATAA814600.029.066685
GGCGCAC107850.028.7536265
ATATAAT811550.028.7377326
AAATATA849700.028.1344784
GCGACAT89300.027.7881747
CTAGCCC167450.027.5511672
TATAATT680750.027.5096157
CGGTGGC202250.026.960051
GTGGCGC162700.025.4648673
CGCACGC120150.025.3664671
TGCGACA98350.025.2926566
TGGCGCA133000.023.4078144
GGTGGCG215300.022.8657192
TAGCCCT204100.022.6619743
CCTAGCC228350.021.1153261
Produced by FastQC (version 0.11.2)