FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A006.C80FNANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A006.C80FNANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29004874
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT717980.24753770693849592No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA708710.2443416923652211No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA693390.23905982146310997No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG615620.21224708647243218No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT580220.20004224117643124No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA496210.17107814362510246No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT480920.16580661581222522No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA468190.1614176982806407No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA438410.1511504583677902No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC419770.14472395225712753No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT413980.14272773603498504No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT385630.13295351670895036No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC361140.12451010819767738No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA346450.11944544216947812No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG331850.11441180540898058No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG292680.10090717856591964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA135050.038.4096871
TACGTAT144450.036.029952
TCGTTTG279950.035.2058832
ACGTATA154450.033.5819743
GTTTCGG289750.032.351699
CGTTTGG311900.030.245833
GGTTTCG316950.029.6128678
CGTTGAT312550.028.606081
CTCGTTT508700.028.1821521
CGTATAT191850.028.0891674
CCCGATT55950.027.2181571
CTCCGTT194050.026.8540231
CCCGAAT63500.025.3871781
GTGTACG103500.025.0015981
CCCGTAT44500.024.9977231
CGCGTAA4300.024.9013541
CCCGTTT120950.024.6897491
CTCGTGT92650.024.0129241
CCTACGT170800.023.36794724-25
TACGTTC173400.023.03475626-27
Produced by FastQC (version 0.11.2)