FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A006.C80FNANXX.s_8.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A006.C80FNANXX.s_8.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29004874
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA1400100.48271197454607107No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA544600.1877615465593817No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC496150.17105745744663464No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC397370.1370011122958162No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA396500.13670116270803315No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA351040.1210279348222647No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT300060.10345157851745883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT475250.039.1935231
CAAATAT508300.035.3808443
ACAAATA549900.032.3580862
TAATTAT602750.029.1427759
ATATAAT784600.028.8458296
AATATAA797350.028.8080545
AAATATA833400.027.7790134
GCGACAT84300.027.3896127
TATAATT654650.027.3159967
CTAGCCC169600.026.3956622
TGCGACA94250.024.3731066
TAGCCCT206000.021.847023
GCGCACG79150.020.9777166
CCTAGCC227000.020.8483731
CGCACGC92900.020.0567021
CCTACAC290000.019.5420341
GCCCTAC230150.019.474765
ATTATAC459550.019.09873610-11
GGCGCAC90800.018.4208055
TATACTA479900.018.4184812-13
Produced by FastQC (version 0.11.2)