FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A007.C80FNANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A007.C80FNANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25606654
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG765630.29899650301831704No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT683300.2668447037242742No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA604330.236005063371419No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA595750.23265437178945753No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA419670.1638909948953112No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT406440.15872436906438459No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT404870.15811124717817487No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA397410.15519794190994263No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC349250.13639033041958548No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA323040.12615470963133255No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT319580.12480349834070473No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA283890.11086571482552933No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT277430.10834293305169819No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG260060.10155953995395105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG243800.038.474392
GTACGTA112850.036.8991241
GTTTCGG246000.035.3730749
TACGTAT121500.034.709132
CTCGTTT437450.032.4324341
GGTTTCG269900.032.1966678
CGTTTGG276750.031.8091533
ACGTATA132950.031.3175413
CGTTGAT253350.030.265761
GAGTCTT357400.029.3241274
GGAGTCT352100.029.2421343
AGTCTTG377550.028.3577545
CCCCGTT51250.028.2053811
CTCCGTT190750.028.0047281
TGGAGTC384450.026.8895572
CTCGTGT86100.026.8069931
CCTACGT196500.026.8051124-25
CCCGTAT39950.026.5046941
TACGTTC199800.026.40698626-27
CCCTACG200350.025.94858224-25
Produced by FastQC (version 0.11.2)