FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A007.C80FNANXX.s_8.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A007.C80FNANXX.s_8.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25606654
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA1234650.4821598323623227No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA443470.17318545406205746No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC394710.15414352847505963No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA324490.12672096869821414No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC322240.12584229083581167No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA296970.11597376213229578No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT276950.10815548177438568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT417250.039.761271
CAAATAT434650.036.087953
ACAAATA475450.032.61572
TAATTAT500300.030.6383939
GCGACAT82550.029.6288247
AATATAA670500.029.5523015
ATATAAT666250.029.2623426
GCGCACG79000.029.0025426
AAATATA694500.028.7391034
TATAATT562700.027.909517
GGCGCAC85550.026.6474115
TGCGACA91900.026.4208666
GGCGATC33550.026.0824642
CTAGCCC151950.025.8955172
CGGTGGC168250.022.5020261
CGCACGC93600.021.9347637
GTGGCGC130950.021.7746543
TAGCCCT185100.021.3220213
TGGCGCA112500.020.4242764
ATTATAC381750.020.20091810-11
Produced by FastQC (version 0.11.2)