FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A012.C80FNANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A012.C80FNANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27573425
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT643640.23342765724606213No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG640010.23211117226097228No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA634190.23000044426834898No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA609570.2210715571242963No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA433970.1573870493056267No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT412620.14964408665227477No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT387390.14049397200384064No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA373330.13539485936186743No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC353120.12806533827408095No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT344740.1250261800991353No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA298480.10824915657013956No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA297690.10796264881856353No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT277570.10066576785437427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA119800.039.6745951
TCGTTTG247350.037.3928222
TACGTAT130950.036.9733352
GTTTCGG249300.035.024699
ACGTATA139150.034.196843
GGTTTCG271150.032.3339848
CGTTTGG276950.031.936613
CTCGTTT443950.031.8506031
CGTTGAT267450.030.3162691
CTCCGTT192350.028.7616161
CGTATAT177050.027.5819424
CGCGTAA4850.026.983821
CCCGTAT45600.026.4821781
CCTACGT167350.026.10674524-25
GTGTACG86100.025.9780881
CCCGAAT61050.025.7241341
CCCGTTT128450.025.4249861
TACGTTC172700.025.36685826-27
GGAGTCT339550.025.1903573
CCCTACG174700.024.80409224-25
Produced by FastQC (version 0.11.2)