FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A012.C80FNANXX.s_8.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A012.C80FNANXX.s_8.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27573425
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA1225580.44447869642599713No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA440640.15980604513222424No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC401210.14550604431622113No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA339580.12315481301289194No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC324040.11751895167176367No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA287190.10415463439888226No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT281010.10191334591186986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT435450.037.7720831
CAAATAT453100.035.0792473
ACAAATA495050.031.2771722
TAATTAT522650.029.1908139
ATATAAT692900.028.386036
AATATAA711750.028.14915
TATAATT577550.027.2940867
AAATATA743950.027.2620354
GCGCACG83700.024.6010936
GCGACAT82150.023.9770977
GGCGCAC90500.022.9538065
CTAGCCC152850.022.5501442
TGCGACA89750.021.417656
CGGTGGC180900.020.501131
CGCACGC100900.019.6984217
ATTATAC390700.019.55493510-11
ATAATTA793000.019.5180058
GTGGCGC141900.019.0041683
TAGTGCG150950.018.779278-79
TATACTA418100.018.5148812-13
Produced by FastQC (version 0.11.2)