FastQCFastQC Report
Wed 4 Nov 2015
SLX-10776.C80FNANXX.s_8.r_1

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.C80FNANXX.s_8.r_1
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190412206
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT4859830.2552268104073118No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG4695830.24661391717713727No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA4544600.23867167423080013No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA4504390.23655993986015791No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT3454310.18141221471905009No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA3077230.16160886240664635No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT3012660.15821779828547336No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA2916030.15314301857308454No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC2646450.1389853127377769No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA2564420.13467729059344022No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT2503850.13149629703885685No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT2317730.12172171357544169No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC2089790.10975084233833202No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG2049720.10764646043752049No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA2003420.1052148936292456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA862500.038.467961
TCGTTTG1777000.038.227022
TACGTAT920400.036.327742
GTTTCGG1848100.034.5961049
ACGTATA990750.033.496823
CTCGTTT3218600.030.6446481
GGTTTCG2119600.030.2571458
CGTTGAT1926500.030.1859191
CGTTTGG2168250.029.9980983
CTCCGTT1353800.028.8520261
CGTATAT1236800.027.8652554
CTCGTGT616200.027.4280951
CCTACGT1233100.025.79160924-25
CCCGTAT263300.025.6082571
AGTCTTG2551800.025.3919355
GAGTCTT2490650.025.3420564
TACGTTC1260350.025.28824826-27
GGAGTCT2483800.025.1706523
CCCGATT357500.024.7929271
TGGAGTC2554350.024.6728172
Produced by FastQC (version 0.11.2)