FastQCFastQC Report
Wed 4 Nov 2015
SLX-10776.C80FNANXX.s_8.r_2

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.C80FNANXX.s_8.r_2
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190412206
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA9083480.47704294755137705No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA3420280.17962503937378888No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC3066870.1610647796391792No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC2442370.12826751243037435No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA2430050.12762049508527828No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA2193810.11521372742249517No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT1953420.1025890115468753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT3001250.040.2131
CAAATAT3195300.036.3086853
ACAAATA3575800.031.922562
TAATTAT3857600.029.4085339
ATATAAT4935000.029.1696936
GCGCACG558600.028.9460836
AATATAA5180300.028.2016665
GCGACAT556800.027.8408137
AAATATA5297150.027.6950384
TATAATT4232550.027.313487
CTAGCCC1069750.026.0782342
GGCGCAC626250.025.7586785
CGCACGC683050.022.0766581
TAGCCCT1269200.021.9002323
GGCGATC236650.021.357942
CGGTGGC1337650.021.2288531
CCTAGCC1383350.020.8890361
CCTACAC1791700.019.8429011
GCCCTAC1399150.019.6212675
ATAATTA5944750.019.2559248
Produced by FastQC (version 0.11.2)