FastQCFastQC Report
Wed 11 Nov 2015
SLX-10777.A002.C8143ANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10777.A002.C8143ANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38332054
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36050.033.5093271
TCTCGTA37350.031.45656641
CGATGTA39250.030.26917534
CTCGTAT39250.030.2137142
CACCGAT39950.029.96147331
ACCGATG40200.029.82937232
ATCGGAA41800.029.0580522
CGTATGC40600.029.0424444
TCGTATG41550.028.64665243
ACACGTC43950.027.72713513
CGTCTGA43850.027.53955516
CCGATGT43950.027.48416933
GTCACCG43750.027.45972629
TATCTCG43150.027.38130639
ACGTCTG46650.026.26380515
TCGGAAG49150.024.8862363
ATCTCGT48250.024.53271540
TCACCGA51300.023.67572830
CGGAAGA53100.022.951994
CACGTCT62750.019.80563714
Produced by FastQC (version 0.11.3)