FastQCFastQC Report
Wed 11 Nov 2015
SLX-10777.A004.C8143ANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10777.A004.C8143ANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32373256
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA33000.033.671741
CTCGTAT33150.031.52574542
TCTCGTA34300.030.72567241
ATCGGAA36800.030.6735272
CGTATGC36350.028.98820544
CGTCTGA39400.028.36108216
TCGTATG37300.028.1949743
ACACGTC41400.026.9378513
AATCTCG39250.026.62649239
TCGGAAG42900.026.1528593
ACGTCTG44150.025.50906815
ATCTCGT42300.025.01869640
CGGAAGA47400.023.4842014
CACGTCT55300.020.44529214
GCACACG60400.018.9374611
CACACGT63350.018.19449412
ACCAATC87150.012.79934736
GACCAAT94450.012.11289435
ACTGACC100400.011.41768632
CCAATCT99050.011.30637337
Produced by FastQC (version 0.11.3)