FastQCFastQC Report
Wed 11 Nov 2015
SLX-10777.A005.C8143ANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10777.A005.C8143ANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23092702
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22600.033.005721
TCTCGTA23400.031.21651541
CTCGTAT24200.030.2748942
ATCGGAA25350.030.033142
CGTATGC25000.029.38919844
TCGTATG25600.028.79043643
CGTCTGA26550.028.25159316
GATCTCG26200.027.71244439
ACACGTC27550.027.06644413
ACGTCTG30400.024.8183915
ATCTCGT29800.024.6599640
TCGGAAG31600.024.2270093
CGGAAGA34550.022.2855284
CACGTCT38050.020.291114
GCACACG42100.018.33910411
CACACGT43750.017.49662412
AGTGATC61700.012.01670236
GTGATCT66800.011.26449737
GTCACAC74350.010.6834629
GAACTCC75950.010.1370221
Produced by FastQC (version 0.11.3)