FastQCFastQC Report
Wed 11 Nov 2015
SLX-10777.A006.C8143ANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10777.A006.C8143ANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33986606
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC510620.1502415392699112TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA64850.036.947461
CTCGTAT63000.036.9139342
TCTCGTA64900.035.76586541
ATCGGAA67550.035.634042
CGTATGC68250.034.00476544
TCGTATG68550.033.95681843
CGCCAAT69300.033.81333
TATCTCG68600.033.77265539
ACGCCAA72050.032.64515332
CGTCTGA73050.032.6110716
ACACGTC74500.032.3011413
ATCTCGT74050.031.34644740
ACGTCTG77300.031.074215
GTCACGC75950.030.91162929
TCGGAAG80650.029.7322313
TCACGCC81700.028.95153830
CACGCCA84650.027.994531
AGTCACG84650.027.83862128
CACGTCT88450.027.53002414
CGGAAGA86900.027.5177674
Produced by FastQC (version 0.11.3)