FastQCFastQC Report
Wed 11 Nov 2015
SLX-10777.A007.C8143ANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10777.A007.C8143ANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39007677
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC650950.1668774072344785TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA77650.037.7148321
TCTCGTA78200.036.63253441
CTCGTAT78750.036.4041242
ATCGGAA83700.035.1206632
CGTATGC82150.034.972844
TCGTATG82400.034.81776443
CGTCTGA88400.033.04447616
ATCTCGT87850.032.8089540
ACACGTC91100.032.37900513
ACGTCTG93300.031.59193815
CATCTCG94650.030.40534239
TCGGAAG98750.029.785033
CGGAAGA101850.028.986244
CACGTCT106000.028.01437814
CACACGT120400.024.88305512
GCACACG124100.024.229811
GATCATC128000.022.929836
AGATCAT161100.018.4234235
GAACTCC174450.017.27514321
ACCAGAT173700.017.23993332
Produced by FastQC (version 0.11.3)