FastQCFastQC Report
Wed 11 Nov 2015
SLX-10777.A012.C8143ANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10777.A012.C8143ANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36042957
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA49400.035.4103281
CTCGTAT49400.033.9822742
TCTCGTA49850.033.5875741
ATCGGAA53500.032.6147962
TCGTATG52750.031.7819243
CGTATGC53150.031.70373744
CGTCTGA57550.029.96565216
AATCTCG56200.029.87082939
ACACGTC60200.029.01195713
ATCTCGT58550.028.67191540
ACGTCTG62550.027.81648315
TCGGAAG63000.027.5866663
CGGAAGA66000.026.565764
CACGTCT72550.024.10364514
CACACGT84750.020.84149412
GCACACG85450.020.74798811
GTAATCT103500.016.8574537
TGTAATC121700.014.73342736
CCTTGTA129550.013.8756733
AGTCACC133600.013.47212328
Produced by FastQC (version 0.11.3)