FastQCFastQC Report
Tue 12 Jul 2016
NF14_ES_+Ng.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF14_ES_+Ng.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22417246
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC9993984.458165824651253TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAACCTCGTATGC657630.29335896122119554TruSeq Adapter, Index 7 (97% over 35bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC281670.12564879735896195TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACTCAATCTCGTATGC229810.10251482273959968TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC225270.10048959626887263TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATC254.445543E-543.99990543
CTCGTAC1200.043.999942
CCCTATG351.4477155E-743.999943
CCGCGTA454.802132E-1043.999941
CCTATGC2200.043.999944
CGCCCCT207.859689E-443.999943
TCCCGTA2900.043.99989741
ACCATTC207.860466E-443.9990232
CCCACCA207.860466E-443.9990229
CACCACA650.043.99901631
TCCAATC454.802132E-1043.9989225
ACCCAGT454.802132E-1043.9989223
CTGACCT2400.043.99872619
TCCGAAC2850.043.99872218
GCCCACG2250.043.99862711
CAAACAC254.4463042E-543.99862738
ACACCGT408.3273335E-943.99862713
GCAACAC408.3273335E-943.99862711
CAGCACA454.802132E-1043.9986279
CAACACG408.3273335E-943.99862712
Produced by FastQC (version 0.11.5)