FastQCFastQC Report
Tue 12 Jul 2016
NF14_ES_N_minus_minus.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF14_ES_N_minus_minus.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28966216
Sequences flagged as poor quality0
Sequence length50
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC6018052.0776099991797343TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATACCTCGTATGC493720.1704468405538369TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTA502.7284841E-1143.9997741
TCCCTTG302.5299396E-643.99976744
ACGATCC600.043.9993135
CCCCCGA454.802132E-1043.9993132
ACTCCAC1850.043.99885623
CTGACCT1150.043.99878319
ACGTCTC900.043.9987815
ACCCGCC207.860781E-443.99870313
ATCACAC502.7284841E-1143.9986310
GCACACC1750.043.9986311
CCCACGA5600.043.6064632
GATCGGA913800.043.3873021
CCCCACG5600.043.2136131
TCCCCAC5400.043.1845130
TCCGAAC2450.043.10084518
CCCGTAT4400.042.99977542
ATCGGAA918200.042.9665832
CGGAAGA916250.042.9187664
TCGGAAG923650.042.848823
AGTCCCC5350.042.76502228
Produced by FastQC (version 0.11.5)