FastQCFastQC Report
Tue 12 Jul 2016
NF17+E14_EV.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF17+E14_EV.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26391200
Sequences flagged as poor quality0
Sequence length50
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC13111494.968129528024493TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGACCTCGTATGC1556040.5896056261177969TruSeq Adapter, Index 7 (97% over 35bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC384750.14578723210767225TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAGCTCGTATGC362590.13739049380096396TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAACTCGTATGC326140.12357907181181607TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACCGGATCTCGTATGC308840.11702385643699414TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC290790.11018445542453545TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCACCGGATCTCGTATGC286930.10872184667616479TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCACCGGATCTCGTATGC283120.1072781836369699TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCACCGGATCTCGTATGC276470.10475840431659039TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCACCGGATCTCGTATGC276220.10466367577071146TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCACCGGATCTCGTATGC275460.10437570099123951TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT207.8595366E-444.00010342
CCACTGG302.530127E-643.9992733
CCCGGAC551.8189894E-1243.9992735
CCACCGT351.4478974E-743.9992733
CCGAACC551.8189894E-1243.99902319
CACGACT207.86049E-443.9990214
CCTCCGA408.3255145E-943.9990216
GCCCCAG302.5302597E-643.9989423
CGAACCC551.8189894E-1243.9989420
ACCCTAG254.4461394E-543.9989423
GAACCCT254.4461394E-543.9989421
CCGGCAC600.043.9986936
CACCTCG351.4480429E-743.9986939
CTGTCAC207.8607834E-443.99868827
CGGCACT502.7284841E-1143.99868837
GAACCCG1250.043.99868839
GGACCCC3300.043.99868838
CGGAGCC551.8189894E-1243.99868837
GACACAC2200.043.9986049
GATCGGA2048250.043.7723961
Produced by FastQC (version 0.11.5)