FastQCFastQC Report
Tue 12 Jul 2016
NF17_ES_+Ng.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF17_ES_+Ng.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20162800
Sequences flagged as poor quality0
Sequence length50
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC2828991.4030739778205408TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCACCTCGTATGC267100.13247168052056263TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTA254.4456756E-543.99965741
ACACCCC207.85989E-443.99965342
CTCCCTC207.85989E-443.99965343
CACCCCC351.44777E-743.99965343
CCCGCAT207.85989E-443.99965342
ACCCTGG551.8189894E-1243.9993332
CCCCATG3100.043.9993331
ATGGCCC600.043.99932535
CACCACG207.860178E-443.99932531
CCCTGGC600.043.99932533
TCCGAAC2200.043.99899718
ACCTCTG408.3255145E-943.99899715
CACGTCC2150.043.99899714
CGTCCGA2150.043.99899716
CTCCCAG254.446131E-543.9988924
CTCCATC207.860562E-443.9988924
GAACCTC207.860562E-443.9988921
GAACCCA207.860562E-443.9988921
ACTCCCA254.446131E-543.9988923
ACTCCAC900.043.9988923
Produced by FastQC (version 0.11.5)