FastQCFastQC Report
Tue 12 Jul 2016
NF17_ES_N_minus_minus.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF17_ES_N_minus_minus.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23488604
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC5810082.473573993584293TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGACCTCGTATGC531260.22617776688644417TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGTA351.4477519E-743.99974441
CCAGGCA454.802132E-1043.99899326
CTCAACT650.043.998919
CTCCAAT650.043.998924
CCTCTGA1500.043.998916
CACCTCT1600.043.99889814
CACGTCA207.8605785E-443.99889814
ACACCTC1700.043.9988113
AGGCACC454.802132E-1043.9988128
AGCCCGT351.4480247E-743.99871440
GCACCAC302.5303361E-643.99871411
GCACAAC454.802132E-1043.99871411
GCCCTCG551.8189894E-1243.99871439
GAGCCCG351.4480247E-743.99871439
CCCCTCT207.860743E-443.9987138
GATCGGA905850.043.5344351
ATCGGAA906550.043.3296782
CGGAAGA908850.043.152164
TCGGAAG913800.043.1375773
ACCTCGT74650.043.11461640
Produced by FastQC (version 0.11.5)