FastQCFastQC Report
Tue 12 Jul 2016
NF17_Ng+Prd.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF17_Ng+Prd.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27727917
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC9987173.601846471193635TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGACCTCGTATGC872430.314639574260122TruSeq Adapter, Index 7 (97% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC291030.10495920050539678TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGC1050.044.0461731
CTCCCCG207.8594725E-444.00018743
TTGCCCC207.8594725E-444.00018742
CTCCTAC207.8594725E-444.00018742
CCGCGTA302.5297722E-644.00018341
CCGGAAG1200.043.999393
GTCCACC302.5301815E-643.9991529
CCAGGTC254.44616E-543.99891326
TCCGATC254.4462548E-543.9987566
ACCCTAG207.860729E-443.99875623
CACGTCA207.860799E-443.9986814
CGCCCGA207.860799E-443.9986816
CATGTCC254.446302E-543.99867614
CACGGCT600.043.99867214
GTCCGAC302.5303689E-643.99867217
AGCCCAC1850.043.9985210
ACACCGT800.043.9985213
CTTCACC254.4463966E-543.9985240
CAGCACA750.043.998529
AACGATC502.7284841E-1143.9985236
Produced by FastQC (version 0.11.5)