FastQCFastQC Report
Tue 12 Jul 2016
NF17_Prd+EV.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF17_Prd+EV.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23266981
Sequences flagged as poor quality0
Sequence length50
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC5974112.5676343656274097TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAACCTCGTATGC357150.15350079152942103TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAT950.043.99968342
CTCGTAC502.7284841E-1143.9996842
CGCATGC1100.043.9996844
TCTCGCA408.3255145E-943.9996841
CTCGCAT800.043.9996842
TCCCCCG254.4456814E-543.9996844
CCCCCAC207.8600546E-443.99949331
ACGTCCG2100.043.99902315
CTGACTC408.3255145E-943.9990219
CCGAACC207.860471E-443.9990219
CTCCAGA254.446189E-543.9988324
AGGCACC502.7284841E-1143.9988328
ACCCGTC1150.043.9988313
AACTCCC3000.043.9988322
TCCGCGC207.860803E-443.99864239
AGCCCGT351.4480429E-743.99864240
GCAACAC207.860803E-443.99864211
AGCCACG454.802132E-1043.99864210
CAACACG207.860803E-443.99864212
CCCACGT850.043.99864212
Produced by FastQC (version 0.11.5)