FastQCFastQC Report
Tue 12 Jul 2016
NF17_ng+EV.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNF17_ng+EV.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21334157
Sequences flagged as poor quality0
Sequence length50
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC1779170.8339537390673557TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG207.8595866E-444.00000843
CTCCCCG254.445474E-544.00000843
CTCCCAC207.8595866E-444.00000843
CTCGCAT254.445474E-544.00000842
CACCCTT207.8595866E-444.00000843
CCGGAAG351.4478792E-743.9992873
GAGACCG254.4459048E-543.99928730
CGAGACC207.8602217E-443.99928729
GGACTCC1250.043.9990821
CGTCCGA950.043.9990816
TCCGAAC1000.043.99907718
CTGACCT254.4460277E-543.99907719
CACCTCT502.7284841E-1143.99897414
ACACCGT207.860675E-443.9987713
TCCCTCG207.860766E-443.9986738
CCTCACC302.5303434E-643.9986740
GCACCCG502.7284841E-1143.99866511
GCACCTC23300.043.33774638
CACCTCG23200.043.33489639
ACCTCGT23100.043.33202440
Produced by FastQC (version 0.11.5)