FastQCFastQC Report
Tue 12 Jul 2016
Nf14.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNf14.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20755188
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC5621752.7085998931929693TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAACCTCGTATGC357890.17243399577975396TruSeq Adapter, Index 16 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA1400.044.042181
TCCCTAT207.8596314E-443.9999544
GCGCAAA207.8602845E-443.9992134
CGCTCAC207.8602845E-443.9992135
CCCGTCC408.3255145E-943.99926
CCAGACA254.4460714E-543.99899726
CGTCCCG408.3255145E-943.9988928
CCGATCT207.860657E-443.9987879
AGCCCAC800.043.99878710
CCCACGT750.043.99878712
GCACCAC207.860657E-443.99878711
CACTCGT302.5302943E-643.99878740
AAGCCCG207.860657E-443.99878739
GAGCCCA850.043.9987879
GAGCCAC750.043.9987879
GAGCACC1350.043.9987879
CACCCGT700.043.99878312
GATCGGA844800.043.73721
CCCGCGC11700.043.62314631
TCCCGCG11350.043.61155330
Produced by FastQC (version 0.11.5)