Bismark

Bismark Processing Report

Nf14.r_1_trimmed.fq

Data processed at 16:48 on 2016-07-20


Alignment

Sequences analysed in total 19955435
Single-end alignments with a unique best hit 14017231
Sequences without alignments under any condition 2094098
Sequences that did not map uniquely 3844106
Genomic sequence context not extractable (edges of chromosomes) 10

Cytosine Methylation

Total C's analysed 124189561
Methylated C's in CpG context 802253
Methylated C's in CHG context 1088930
Methylated C's in CHH context 3662255
Methylated C's in Unknown context 1
Unmethylated C's in CpG context 4409090
Unmethylated C's in CHG context 25850368
Unmethylated C's in CHH context 88376665
Unmethylated C's in Unknown context 19
Percentage methylation (CpG context) 15.4%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 4.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7102131 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6915090 original bottom strand

Deduplication

Alignments analysed 14017221
Unique alignments 11076867
Duplicates removed 2940354
Duplicated alignments were found at 2049674 different positions

Cytosine Methylation after Extraction

Total C's analysed 98710682
Methylated C's in CpG context 448934
Methylated C's in CHG context 864771
Methylated C's in CHH context 2915484
Unmethylated C's in CpG context 3334225
Unmethylated C's in CHG context 20587566
Unmethylated C's in CHH context 70559702
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 4.0%

M-Bias Plot