FastQCFastQC Report
Tue 12 Jul 2016
NfV_+2.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNfV_+2.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21967406
Sequences flagged as poor quality0
Sequence length50
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC2983721.3582486707807013TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACA207.8604126E-443.99907731
TCACTAC207.8604126E-443.99907730
CGAGACC207.8604126E-443.99907729
TCCCCTA207.8604126E-443.99907744
CCTATGC551.8189894E-1243.99907744
CCCTATA4200.043.99907331
CTCGTAC302.5302252E-643.99897842
TTGCTCC207.8605005E-443.99897842
CCCGTCC207.8605005E-443.99897826
TCCCGTA1350.043.99897841
TGCGTCC207.8605005E-443.99897841
ACGCCTG408.3255145E-943.9988815
CGTCCGA1100.043.9988816
CACGTCG351.4479701E-743.99887514
CACGTCC1250.043.99887514
GACCTCC600.043.9987821
ACACGCT502.7284841E-1143.99877513
CAGCACA207.860677E-443.9987759
CTCCCAG408.3255145E-943.99877524
CTCCAGG351.4480065E-743.99877524
Produced by FastQC (version 0.11.5)