FastQCFastQC Report
Tue 12 Jul 2016
NfV_+3.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNfV_+3.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20411631
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC8391254.111013960618825TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAACCTCGTATGC469590.230060008433427TruSeq Adapter, Index 18 (97% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC244910.1199855121817556TruSeq Adapter, Index 19 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCC254.4457513E-543.9995342
CTCCCTA207.86E-443.9995343
GCTCGCA207.86E-443.9995341
CACGGGC454.802132E-1043.9994231
CACGAGC302.5300415E-643.9994231
CGCTAAC302.5300415E-643.9994235
CGAACCC254.4460725E-543.99899320
ACCCAGT408.3255145E-943.99899323
CACGACT254.446137E-543.99888614
CCGAACC254.446137E-543.99888619
CACGTCA207.860569E-443.99888614
CCGAACT2500.043.99888219
TCCGAAC2750.043.9988818
AGCACCG408.3273335E-943.9986710
CTTCGCC207.860759E-443.9986740
CAGCACA454.802132E-1043.998679
CTAAGCC454.802132E-1043.9986737
AAGCCCG408.3273335E-943.9986739
ACAGCAC454.802132E-1043.998678
CACACGA254.4462653E-543.9986712
Produced by FastQC (version 0.11.5)