FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPI31.C80Y3ANXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPI31.C80Y3ANXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36186628
Sequences flagged as poor quality0
Sequence length50
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC26238047.250755721146497TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCACGATATCTCGTATGC608820.16824446864736886TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCACGATATCTCGTATGC564080.15588078557637366TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCACGATATCTCGTATGC560310.15483896427155358TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACGATATCTCGTATGC558550.15435259676585505TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCACGATATCTCGTATGC530700.14665638367852346TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCACGATATCTCGTATGC526080.14537966897606486TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCACGATATCTCGTATGC490300.13549203866135304TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATTTCGTATGC385420.10650895684450068TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCACGATATCTCGTATGC383500.1059783741110114TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3423050.043.6317371
TCGGAAG3464450.043.4430543
ATCGGAA3466650.043.4237332
CGGAAGA3461550.043.382274
AAGAGCA3448500.043.3661847
AGAGCAC3407250.043.3592228
GAGCACA3397200.043.358649
GAAGAGC3460100.043.35436
GAATTCC58750.043.24583421
AGCACAC3348850.043.2417310
CACACGT3338100.043.22744812
GCACACG3340250.043.20489511
ACACGTC3290850.043.16028613
CACGTCT3296650.043.11771414
ACGTCTG3332550.043.09283415
CGTCTGA3332850.043.06717716
CACATGT58950.043.02430312
GTCTGAA3383350.043.01795217
ACACGTT71500.043.01000213
TCTGAAC3334050.042.99841318
Produced by FastQC (version 0.11.3)