FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPI31.C810LANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPI31.C810LANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881480
Sequences flagged as poor quality0
Sequence length50
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC215812.4482688206198664TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCACGATATCTCGTATGC19690.22337432499886556TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCACGATATCTCGTATGC18440.2091936288968553TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACGATATCTCGTATGC17830.20227344919907428TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCACGATATCTCGTATGC17300.19626083405182193TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCACGATATCTCGTATGC17020.19308435812497163TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCACGATATCTCGTATGC16440.1865045151336389TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCACGATATCTCGTATGC11860.1345464446158733TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG2050.043.9990423
TCTAGTC2050.043.9990425
CTAGTCA2050.043.9990426
AGCATAC1200.043.9990410
CATACGT1200.043.9990412
ATACGTC1200.043.9990413
CTCTAGT2050.043.9990424
AGAGCAT1150.043.999048
TACGTCT1200.043.9990414
GCATACG1200.043.9990411
GTCATCA750.043.99903529
AGTCATC750.043.99903528
AGTCACT207.856331E-443.99903528
TCATCAC750.043.99903530
AAGGGCA207.856331E-443.9990357
GGGCACA207.856331E-443.9990359
AACTCCG351.4458237E-743.99903522
GCGTCTG207.856331E-443.99903515
GATCGGA39300.043.629461
AAGAGCA38500.043.370487
Produced by FastQC (version 0.11.3)