FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPI44.C80Y3ANXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPI44.C80Y3ANXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53389357
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC21953784.112014310267869TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2663050.043.6781779
CACACGT2630300.043.67674612
AGAGCAC2670450.043.6741148
ACACGTC2609150.043.66658413
CACGTCT2611500.043.6508714
ACGTCTG2628650.043.63219515
CGTCTGA2627850.043.61538316
AGCACAC2641750.043.55821610
TCTGAAC2625800.043.55728518
GCACACG2639050.043.5552711
CTGAACT2621600.043.5230619
GTCTGAA2655950.043.51752517
AAGAGCA2698050.043.507817
GAAGAGC2707050.043.500496
TGAACTC2618000.043.48964320
GAACTCC2592900.043.47287821
CTCCAGT2584250.043.43704224
ACTCCAG2588650.043.42949323
TCCAGTC2581150.043.428725
AACTCCA2590100.043.42560622
Produced by FastQC (version 0.11.3)