FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPI44.C810LANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPI44.C810LANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3961660
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC735491.8565197417244286TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA551.8189894E-1243.999833
TCCGGTC254.4449404E-543.99979825
TGAGCTC408.3236955E-943.9986920
GAACTTC2750.043.99868821
CGCGTCT600.043.99868814
CACACGT92650.043.50005312
ACGTCTG94050.043.48408515
CACGTCT92050.043.472914
AGAGCAC95850.043.4707958
CGTCTGA93850.043.41266616
ACACGTC91800.043.3995813
GAGCACA95600.043.3313459
GCACACG93100.043.31342311
TCTGAAC94200.043.29807318
CTGAACT93950.043.29620719
TGAACTC93400.043.17429720
AGCACAC93500.043.15165310
GAACTCC91650.043.1345621
GTCTGAA96700.043.11143517
CTCCAGT92500.043.0960224
Produced by FastQC (version 0.11.3)