FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPIBS41.C80Y3ANXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPIBS41.C80Y3ANXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38229528
Sequences flagged as poor quality0
Sequence length50
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC22977666.010448258738638TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCGCTAATCTCGTATGC504540.13197651825573156TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCGCTAATCTCGTATGC470990.12320057940553175TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCGCTAATCTCGTATGC444730.11633154351264813TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC444050.11615367053446227TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCGCTAATCTCGTATGC410120.10727833207880569TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCGCTAATCTCGTATGC393500.10293090722961581TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCGCTAATCTCGTATGC386480.10109463030775583TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2941950.043.5401151
AGAGCAC2916350.043.4031878
AAGAGCA2951100.043.3982287
GAGCACA2909200.043.3941739
CGGAAGA2968700.043.387454
GAAGAGC2961800.043.384786
ATCGGAA2978000.043.383542
TCGGAAG2978100.043.378393
CACATGT48100.043.3085512
CACACGT2865150.043.2628712
AGCACAC2872450.043.2448210
GCACACG2868250.043.21534311
ACATGTC47800.043.21220813
ACACGTC2832500.043.20395713
CACGTCT2837450.043.17122714
CAGTTAC47750.043.1653227
ACGTCTG2868900.043.14881515
CGTCTGA2868800.043.12271516
GTCTGAA2908850.043.09789317
TCTGAAC2864650.043.0594818
Produced by FastQC (version 0.11.3)