FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPIBS41.C810LANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPIBS41.C810LANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838704
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC215200.7580924252757596TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCGCTAATCTCGTATGC28520.10046838275494734TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTC408.3200575E-943.999725
GAACTCT2250.043.99892821
GCACACT302.529463E-643.99892811
ACATGTC2600.043.99892813
TGGACTC254.4451397E-543.99892420
CTGCACT207.8593846E-443.99892419
TGCACTC207.8593846E-443.99892420
TCTGGAC254.4451397E-543.99892418
TGTCTGA2700.043.1841316
CACATGT2650.043.1687612
TCTAGTC2350.043.06353425
ACTCTAG2300.043.0424323
TCCAGTT2000.042.89970825
CTTCAGT3000.042.53229524
AGCACAC40250.042.4138710
CACACGT40150.042.35513312
AAGAGCA45450.042.3532037
AGAGCAC43300.042.3222928
GAGCACA42900.042.3066649
GCACACG40250.042.30455811
Produced by FastQC (version 0.11.3)