FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPIBS49.C80Y3ANXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPIBS49.C80Y3ANXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37826830
Sequences flagged as poor quality0
Sequence length50
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC30028547.938423600391574TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGCTAATCTCGTATGC564060.14911638115062772TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC480850.1271187672876633TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGCTAATCTCGTATGC466150.12323263672900954TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGCTAATCTCGTATGC458310.1211600337643942TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATTTCGTATGC441010.11658656038584253TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGCTAATCTCGTATGC418130.11053794357074066TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGCTAATCTCGTATGC405440.10718318188439263TruSeq Adapter, Index 19 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3698350.043.6602361
AGAGCAC3688100.043.6168
GAGCACA3680600.043.6110469
CACACGT3629200.043.56804312
GAAGAGC3734750.043.5571146
AAGAGCA3726500.043.550247
AGCACAC3638100.043.5461110
ACACGTC3600200.043.5450613
CGGAAGA3734550.043.5442624
TCGGAAG3743400.043.5393453
GCACACG3633000.043.52852211
CACGTCT3606050.043.51772714
ACGTCTG3636350.043.51019715
ATCGGAA3744850.043.5042722
CGTCTGA3634550.043.49125716
TCTGAAC3642000.043.43985418
CTGAACT3634400.043.4253819
GTCTGAA3683150.043.41135417
TGAACTC3626950.043.4054120
GAACTCC3590950.043.3737821
Produced by FastQC (version 0.11.3)