FastQCFastQC Report
Tue 12 Jan 2016
SLX-10775.RPIBS49.C810LANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10775.RPIBS49.C810LANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752025
Sequences flagged as poor quality0
Sequence length50
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC166660.9512421341019678TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGCTAATCTCGTATGC26930.15370785234228962TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC24180.13801172928468486TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGCTAATCTCGTATGC20230.11546638889285256TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGCTAATCTCGTATGC17650.10074057162426336TruSeq Adapter, Index 19 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTCC254.44436E-543.99905821
CTAACTC207.858441E-443.99905819
TCCGGTC302.5288537E-643.99905825
AGAGCAC35100.042.80828
GAGCACA34900.042.7383429
GAACTTC3350.042.68565421
AACTTCA3350.042.68565422
CACACGT31500.042.67210412
ACTCTAG1650.042.66575223
TCTAGTC1650.042.66575225
CTAGTCA1650.042.66575226
AACTCTA1650.042.66575222
CTCTAGT1650.042.66575224
CACATGT3250.042.6452412
CTGAACT33300.042.61170219
ACGTCTG32050.042.5575915
CACGTCT30450.042.55409614
AGCACAC31950.042.5530810
CGTCTGA31850.042.5485416
ACACGTC30250.042.54454413
Produced by FastQC (version 0.11.3)