Bismark

Bismark Processing Report

cp_SLX-10775.RPIBS41.C80Y3ANXX.s_6.r_1_trimmed.fq

Data processed at 13:36 on 2016-01-25


Alignment

Sequences analysed in total 34586566
Single-end alignments with a unique best hit 24755018
Sequences without alignments under any condition 2733306
Sequences that did not map uniquely 7098242
Genomic sequence context not extractable (edges of chromosomes) 14

Cytosine Methylation

Total C's analysed 231857749
Methylated C's in CpG context 7825117
Methylated C's in CHG context 252148
Methylated C's in CHH context 916950
Methylated C's in Unknown context 54
Unmethylated C's in CpG context 2784835
Unmethylated C's in CHG context 50674586
Unmethylated C's in CHH context 169404113
Unmethylated C's in Unknown context 2130
Percentage methylation (CpG context) 73.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12363743 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 12391261 original bottom strand

Deduplication

Alignments analysed 24755004
Unique alignments 24013535
Duplicates removed 741469
Duplicated alignments were found at 644553 different positions

Cytosine Methylation after Extraction

Total C's analysed 225255839
Methylated C's in CpG context 7583723
Methylated C's in CHG context 245274
Methylated C's in CHH context 892749
Unmethylated C's in CpG context 2642133
Unmethylated C's in CHG context 49268303
Unmethylated C's in CHH context 164623657
Percentage methylation (CpG context) 74.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot