Alignment
| Sequences analysed in total |
49,710,954 |
| Single-end alignments with a unique best hit |
35,647,531 |
| Sequences without alignments under any condition |
3,811,248 |
| Sequences that did not map uniquely |
10,252,175 |
| Genomic sequence context not extractable (edges of chromosomes) |
18 |
Cytosine Methylation
| Total C's analysed |
330,358,805 |
| Methylated C's in CpG context |
11,627,481 |
| Methylated C's in CHG context |
1,865,533 |
| Methylated C's in CHH context |
6,492,945 |
| Methylated C's in Unknown context |
164 |
| Unmethylated C's in CpG context |
3,379,248 |
| Unmethylated C's in CHG context |
70,102,242 |
| Unmethylated C's in CHH context |
236,891,356 |
| Unmethylated C's in Unknown context |
3,029 |
| Percentage methylation (CpG context) |
77.5% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
2.7% |
| Methylated C's in Unknown context |
N/A% |
Alignment to Individual Bisulfite Strands
| OT |
17,798,554 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
17,848,959 |
original bottom strand |
Deduplication
| Alignments analysed |
35,647,513 |
| Unique alignments |
33,355,926 |
| Duplicates removed |
2,291,587 |
| Duplicated alignments were found at 2015798 different positions |
Cytosine Methylation after Extraction
| Total C's analysed |
309,813,301 |
| Methylated C's in CpG context |
10,868,393 |
| Methylated C's in CHG context |
1,745,344 |
| Methylated C's in CHH context |
6,076,463 |
| Unmethylated C's in CpG context |
3,064,912 |
| Unmethylated C's in CHG context |
65,808,583 |
| Unmethylated C's in CHH context |
222,249,606 |
| Percentage methylation (CpG context) |
78.0% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
2.7% |
M-Bias Plot
