Bismark

Bismark Processing Report

cp_SLX-10775.RPI44.C80Y3ANXX.s_6.r_1_trimmed.fq

Data processed at 13:36 on 2016-01-25


Alignment

Sequences analysed in total 49710954
Single-end alignments with a unique best hit 35647531
Sequences without alignments under any condition 3811248
Sequences that did not map uniquely 10252175
Genomic sequence context not extractable (edges of chromosomes) 18

Cytosine Methylation

Total C's analysed 330358805
Methylated C's in CpG context 11627481
Methylated C's in CHG context 1865533
Methylated C's in CHH context 6492945
Methylated C's in Unknown context 164
Unmethylated C's in CpG context 3379248
Unmethylated C's in CHG context 70102242
Unmethylated C's in CHH context 236891356
Unmethylated C's in Unknown context 3029
Percentage methylation (CpG context) 77.5%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 2.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 17798554 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 17848959 original bottom strand

Deduplication

Alignments analysed 35647513
Unique alignments 33355926
Duplicates removed 2291587
Duplicated alignments were found at 2015798 different positions

Cytosine Methylation after Extraction

Total C's analysed 309813301
Methylated C's in CpG context 10868393
Methylated C's in CHG context 1745344
Methylated C's in CHH context 6076463
Unmethylated C's in CpG context 3064912
Unmethylated C's in CHG context 65808583
Unmethylated C's in CHH context 222249606
Percentage methylation (CpG context) 78.0%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 2.7%

M-Bias Plot