Bismark

Bismark Processing Report

cp_SLX-10775.RPIBS49.C80Y3ANXX.s_6.r_1_trimmed.fq

Data processed at 13:36 on 2016-01-25


Alignment

Sequences analysed in total 33496333
Single-end alignments with a unique best hit 23695359
Sequences without alignments under any condition 2893253
Sequences that did not map uniquely 6907721
Genomic sequence context not extractable (edges of chromosomes) 17

Cytosine Methylation

Total C's analysed 212736033
Methylated C's in CpG context 7422868
Methylated C's in CHG context 1460258
Methylated C's in CHH context 4970886
Methylated C's in Unknown context 118
Unmethylated C's in CpG context 2277342
Unmethylated C's in CHG context 44893994
Unmethylated C's in CHH context 151710685
Unmethylated C's in Unknown context 1955
Percentage methylation (CpG context) 76.5%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 3.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11812605 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 11882737 original bottom strand

Deduplication

Alignments analysed 23695342
Unique alignments 20913238
Duplicates removed 2782104
Duplicated alignments were found at 2381384 different positions

Cytosine Methylation after Extraction

Total C's analysed 188523118
Methylated C's in CpG context 6559543
Methylated C's in CHG context 1281081
Methylated C's in CHH context 4357074
Unmethylated C's in CpG context 1938347
Unmethylated C's in CHG context 39840409
Unmethylated C's in CHH context 134546664
Percentage methylation (CpG context) 77.2%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 3.1%

M-Bias Plot