Bismark

Bismark Processing Report

cp_SLX-10775.RPI31.C80Y3ANXX.s_6.r_1_trimmed.fq

Data processed at 13:36 on 2016-01-25


Alignment

Sequences analysed in total 32152792
Single-end alignments with a unique best hit 22956194
Sequences without alignments under any condition 2594644
Sequences that did not map uniquely 6601954
Genomic sequence context not extractable (edges of chromosomes) 26

Cytosine Methylation

Total C's analysed 213576928
Methylated C's in CpG context 7400887
Methylated C's in CHG context 329151
Methylated C's in CHH context 1279594
Methylated C's in Unknown context 58
Unmethylated C's in CpG context 2367053
Unmethylated C's in CHG context 46348119
Unmethylated C's in CHH context 155852124
Unmethylated C's in Unknown context 1953
Percentage methylation (CpG context) 75.8%
Percentage methylation (CHG context) 0.7%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11456216 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 11499952 original bottom strand

Deduplication

Alignments analysed 22956168
Unique alignments 20994285
Duplicates removed 1961883
Duplicated alignments were found at 1727893 different positions

Cytosine Methylation after Extraction

Total C's analysed 195788196
Methylated C's in CpG context 6773758
Methylated C's in CHG context 302543
Methylated C's in CHH context 1177348
Unmethylated C's in CpG context 2092735
Unmethylated C's in CHG context 42532370
Unmethylated C's in CHH context 142909442
Percentage methylation (CpG context) 76.4%
Percentage methylation (CHG context) 0.7%
Percentage methylation (CHH context) 0.8%

M-Bias Plot