FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.H5VHJBBXX.s_3.r_1.lostreads.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.H5VHJBBXX.s_3.r_1.lostreads.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11796383
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1710841.4503089633491892No Hit
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT236340.20034954782326073No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT177620.1505715777454835No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG169600.14377288360338927No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG163220.13836444611878065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155950.13220154008224386No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG145320.12319030333280974No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT140860.11940948339842815No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG137580.11662897008345695No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA136680.115866024356788No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT135270.11467074271834003No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA133250.11295835342070532No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT131860.11178002613173885No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA126960.10762621050876357No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG123060.10432011235986488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG19700.030.0752161
AGTCTTG103500.026.3114645
CGCGGTT5000.026.0113961
TGGAGTC104100.025.2215062
GTCTTGG111600.024.7203336
CCGATTT20050.024.1446881
TCGTTTG108800.023.8675122
CCCGTAT9350.023.1830621
CCCGTTT35500.021.9814621
GAGTCTT124600.021.937874
CGATTTG18900.020.9329172
CCCGAAT15550.020.9094811
CGTTGAT128750.020.3713861
ATGCCGT103000.020.1831744
CGCTGTT110050.020.0905821
CCCGCTA10700.019.5828271
GTTTCGG132300.018.7916229
CCCGATT17950.018.5163141
GGAGTCT144500.018.468643
CGACGGA14150.018.3826121
Produced by FastQC (version 0.11.3)