FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.H5VHJBBXX.s_3.r_2.lostreads.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.H5VHJBBXX.s_3.r_2.lostreads.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11796383
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1690591.4331426844991384No Hit
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC188000.1593708851263985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174810.1481894916433283No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC149020.1263268579868931No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA144850.12279187611999372No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT143350.12152029990887886No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA135990.11528109929967517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC114250.041.033251
TACAACC81350.037.8517572
CTACAAC89000.035.61341
GCGCACG73200.034.1298036
GGCGCAC81500.031.2572425
CGCACGC85300.030.67711
GTGGCGC114050.030.1531313
GCACGCC97050.026.6874332
GCGACAT28400.025.8581357
TGGCGCA102000.024.6842424
CACGCCT108000.024.1990459
GGTGGCG171800.024.1630922
ACGCCTG107750.023.5672724
GCGCCCG42600.021.9709666
CCTTATA61300.020.0840386
CGACAAT102000.019.5118561
TGTAGTC131000.019.1809239
GGCGCCC48950.018.9645275
GCTGCGA37800.018.8406244
ACAACCA172700.018.4132213
Produced by FastQC (version 0.11.3)