FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI01.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI01.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34676488
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT913950.2635647531549331No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG719530.20749794500527274No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT685750.1977564740696924No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA594860.1715456305725078No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG581510.16769575973206974No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT571410.16478312336589565No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA559740.1614177306536925No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG542360.15640568906516716No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT524690.15131001732355365No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA523240.15089186655811282No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG516390.14891646466620265No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT512620.14782927267605647No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG507330.146303743331793No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT468100.1349906022778316No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT454750.13114073143739355No Hit
CTCCTTTGTCCTTTAGTTTCATAAAGGGTTTCGTAATGTTCTCTGGGAAA452380.13045727122077644No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG438340.1264084182919562No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT415080.1197007032546087No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA398270.11485303817387735No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA397350.11458772872270111No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC379360.10939977543285237No Hit
ATTAGGTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGG377300.10880571296608815No Hit
CCCAACCGAAATTTTTTAGTTCATATTTATTTTGTTTTAGCCCATTAGGT374410.10797229523358882No Hit
CCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTG372570.10744167633123632No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC367110.10586712241447288No Hit
CTGAAGATGGCGGTATATAGGCTGAATTAGCGAGAAGGGGTAAGGTAGAA366460.1056796755196201No Hit
ATAAGATATTAGTATTACTTTGACTTGTGAGTCTAGGTTAAAATCATTCG366380.1056566051325613No Hit
CTGATGGTAAGCTTAAAGTTGAACTGAAATTCTTTTTTGGGCAACCAGCT352990.10179519909859383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGA53150.026.3557951
CCGATTT84000.026.131961
GAGTCTT353250.024.6277084
GGAGTCT365300.023.9533273
AGTCTTG373850.023.8093515
CGATGCG50100.023.5746563
CGTTGAT486750.023.260431
GTCTTGG390300.022.5656626
TGGAGTC384000.022.4305132
CCCGCTA38600.022.2606241
CGCTGTT410050.021.9411241
CGCGTTT23550.021.7693331
CCCGATT75300.021.4798221
CCCGAAT59450.021.0122181
TCGTTTG430950.020.8725152
CGATTTG77400.020.7492872
CCCGTAT35850.020.1413461
CGTTTGG440000.020.1158523
TCTTGGA463250.019.7112397
CTGGAGT622250.019.4949471
Produced by FastQC (version 0.11.3)