FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI01.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI01.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34676488
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC650560.18760838756220066No Hit
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC615340.17745164965956184No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA585990.1689877014073628No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG502310.14485607654385302No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT472220.13617872721136004No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT450220.12983437077018872No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA445520.12847898553048395No Hit
TGAAAATTCATTGTTAGGACCTAAGCCCAATAACGAAAGTAATTCTAATC412080.11883556373990353No Hit
TGACAACCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCA402330.1160238603171117No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTA376610.10860673087770595No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCC368560.10628527317991371No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT354830.10232581800094635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC261200.055.721731
GTGGCGC265450.049.840713
TGGCGCA237000.049.294864
GCGCACG264350.044.1840866
GGCGCAC288550.041.0935365
CGCACGC297600.039.0781487
GGTGGCG363500.037.941332
TACAACC272700.035.9512022
GCACGCC334900.033.9731868
CTACAAC310500.033.4009861
CACGCCT393900.029.6340759
TGCGACA87400.025.4590036
GCGACAT93600.024.0803537
ACAACCA447750.022.3781973
ACCAACC460800.021.7374736
CCTTATA234600.021.6092156
GGCCTTA242800.021.1680564
GCCTTAT242000.020.9141865
ACGCCTG396600.020.0376994
CGACAAT370150.019.304631
Produced by FastQC (version 0.11.3)