FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI02.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI02.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56696621
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT1912870.33738694939156955No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG1619730.2856836918023739No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT1507220.265839475689389No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT1304080.23001017997174825No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1133390.19990432939557368No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1101940.19435726160823588No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA1089760.1922089854349521No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT1042310.18383987998156012No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT1031730.18197380757488177No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA982290.17325371118677424No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA974630.1719026606541508No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT968530.17082675879396764No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA933920.16472233856758411No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG930610.16413852952541916No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT875790.15446952297210093No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA834980.14727156315012144No Hit
GTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTT825290.14556246658861735No Hit
ATAAGATATTAGTATTACTTTGACTTGTGAGTCTAGGTTAAAATCATTCG759230.13391097857489603No Hit
ATTAGGTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGG757290.13356880650788697No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTGCACCATTGGGAT754250.13303261935133665No Hit
CCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTG734650.12957562321042027No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG717850.1266124836610633No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC715010.1261115719753387No Hit
CTGATGGTAAGCTTAAAGTTGAACTGAAATTCTTTTTTGGGCAACCAGCT713300.12580996669977915No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT699850.12343769128675235No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG671920.11851147178594647No Hit
CTCGTTTAGCCATTCATTCTAGTCCCTAATTAAGGAACAAGTGATTATGC652090.11501390885358054No Hit
CCCCAACCGAAATTTTTTAGTTCATATTTATTTTGTTTTAGCCCATTAGG646300.11399268397317716No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT624260.11010532708818749No Hit
CCCAACCGAAATTTTTTAGTTCATATTTATTTTGTTTTAGCCCATTAGGT622980.10987956407490315No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC618900.10915994447005933No Hit
GGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTA618110.10902060636029791No Hit
CTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCAAAAGG597130.10532020947068434No Hit
GTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGA578960.10211543294617151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA66350.030.6897621
GGAGTCT620150.029.8559933
GAGTCTT627250.029.1276874
AGTCTTG661600.028.3869975
TGGAGTC660400.027.6328532
GTCTTGG695700.027.1399236
TCGTTTG813250.025.9548152
CGATGCG96800.025.8898813
CCCGATT141650.025.5391541
CGTTTGG826250.025.2936863
CGTTGAT978400.024.9451221
CCCGTTT218600.024.3115711
TCTTGGA792950.024.2651397
CGCTATG125950.024.0215591
CCCGAAT87600.023.7396181
CGTTAGG86250.023.2741
CCCGTAT59650.023.2421421
CTGGAGT1093800.023.0460911
CTTGGAA825900.022.5749238
TTGGAAG838000.022.3434379
Produced by FastQC (version 0.11.3)