FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI02.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI02.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56696621
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC1966070.34677022463119983No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA1518580.2678431224322875No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC1124120.1982693113228035No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT1078510.19022474020100774No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA986520.17399978739473734No Hit
CATAACTAAACCCCCACATAAACTAAAACATTTAACTCAAAAAGTATTGG862420.1521113577474044No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT795440.14029760256788495No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT790750.13947039277702283No Hit
CCTGGCCTTATAATTAATTGGAGGTAAGATTACACATGCAAACATCCATA724420.12777128287768683No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG692490.12213955396036741No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCC682760.12042340230469818No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG676160.11925931176745085No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTA676130.11925402044682698No Hit
CCTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAA623080.109897201810316No Hit
CAATTATCGAATCATAGGTACTAACCCAACAATAGAATTACCTATCCCTA620020.10935748710668312No Hit
CTGTCTCTTACTTTCAATCAGTGAAATTGACCTTCCAGTGAAGAGGCTGG615450.1085514425983164No Hit
CCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAG596910.10528140645277607No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT591510.1043289687404828No Hit
CTGGCCTTATAATTAATTGGAGGTAAGATTACACATGCAAACATCCATAA589590.10399032422055629No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA581600.10258106916107047No Hit
TGACAACCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCA581000.10247524274859343No Hit
CTTACACACACACTAATTCCACAAACCTCAATAAATTCCTATATTACAAA578490.10203253558973117No Hit
CAACAAATCAAAATGTAAACTTAAAATATAGCCAAAAGAGGGACAGCTCT567340.10006592809119964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC486150.068.347411
TACAACC609700.052.808182
GTGGCGC483000.049.4776543
CTACAAC669450.049.1527141
TGGCGCA427200.042.7986644
GGTGGCG755750.039.8693922
GCGCACG467550.039.3660476
ACAACCA872850.037.2831883
GGCGCAC534250.034.713015
TGCGACA170200.034.1007966
CGCACGC553950.033.8133851
GCGACAT176300.033.2476927
GCGCCCG176000.032.977026
ACCAACC988350.032.8808946
GCACGCC610450.030.4220282
GCGGACG112250.029.1245066
CGGACGC110200.028.5554667
GGCCTTA421900.028.3562764
CCTTATA422500.028.0708736
GCCTTAT427900.027.9963875
Produced by FastQC (version 0.11.3)