FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI03.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI03.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31131102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG623180.20017922911948313No Hit
CTCCTTTGTCCTTTAGTTTCATAAAGGGTTTCGTAATGTTCTCTGGGAAA497820.15991081844773758No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG441360.1417746149815063No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC399960.12847601732826547No Hit
GTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCGCGACCGCCCCCCTTTCCG385530.12384078147956344No Hit
CGGCTCCCTCGGCCCCGGGATTCGGCGAGCGCTGCTGCCGGGGGGCTGTA360340.11574919512968092No Hit
CTCGGCTTCGCTCCCCCCCACCCCGAGAAGGGGAGGGAGAGCGCGTGACG358540.11517099523171394No Hit
CGCGCCGTGGGAGGGGCGGCCCGGCCCCCGCGACCGCCCCCCTTTCCGCC342390.10998325725828786No Hit
CCGGCTCCCTCGGCCCCGGGATTCGGCGAGCGCTGCTGCCGGGGGGCTGT342320.10996077170670027No Hit
CGGGTATCCGGCTCCCTCGGCCCCGGGATTCGGCGAGCGCTGCTGCCGGG339440.10903565186995308No Hit
CGACGGAGGCGACGGGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGCGG335800.10786640318739761No Hit
GTGCGCTCGGCTTCGCTCCCCCCCACCCCGAGAAGGGGAGGGAGAGCGCG329190.1057431246731966No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA325380.1045192682224998No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT325220.10446787267601385No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG322270.10352026728767906No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC317940.10212937531090291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT259200.028.05241
CGACGGA237950.023.8209721
AGGCGAC244400.023.3180567
CGGCCGA189300.022.597014
GTTGATC335800.021.938662
CCCGAAT51450.021.472451
TTTGTCC439450.021.2210065
GATCAAT366650.021.0973175
CCTTTGT427450.020.705313
TCCTTTA462500.020.3977859
CGATGCG43600.019.8202863
ATCAATA389200.019.7638636
CGTTCGA28650.019.354474
TGATCAA414850.019.0080784
CGCGTTT19000.019.0016771
CCGCAAC63950.018.9689921
TCGTTTG292650.018.9477392
CGCGGGT243900.018.8583261
TTGATCA406650.018.8246823
CTTTGTC481100.018.8154724
Produced by FastQC (version 0.11.3)