FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI03.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI03.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31131102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT396360.1273196175323315No Hit
CTCCGTCGTCCCTCTCGGGGGTCCGGGGGCCCGGGGGGCGGGGCGGACTG392860.12619533995295124No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC359380.11544082185076518No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA318870.1024281119248525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC305150.041.2732851
CGCACGC281400.028.3526547
GTGGCGC366100.027.7231883
TGGCGCA293600.027.5820064
GCACGCC297700.025.7117988
GCGACAT75100.025.3129087
CACGCCT325850.024.573399
TGCGACA80850.023.1563786
GCGCACG363250.022.2613476
CGACAAT219450.022.1633471
GGCGCAC408300.020.8237955
TCCGTCG319550.019.5075552
ACGCCTG341700.019.4861554
CGGTGCG269950.019.1822361
CGACATC107300.018.5219788
CAATTAG261800.018.3944114
CGCGAAG74000.018.3835981
TCCACCG240700.017.7936343
TGGCGCC122000.017.582714
TGCGCCG299150.017.0121734
Produced by FastQC (version 0.11.3)