FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI04.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI04.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46574467
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT1322570.2839688965200611No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT1017090.21837931070687291No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG945060.20291375529858455No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG914950.1964488396614394No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG808280.17354573268653833No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT799860.1717378751752543No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA778470.1671452300248546No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT754430.16198360359121233No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT753630.16181183565664853No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA739450.15876724901650513No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA733420.15747254820973045No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG727730.1562508487751454No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA688920.14791795684961892No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT684140.14689164344060018No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG661910.1421186419589085No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT626850.13459091222664984No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC621320.13340356637897757No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT597080.12819899796169434No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG571990.12281192611393707No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA559540.12013878763228789No Hit
ATAAGATATTAGTATTACTTTGACTTGTGAGTCTAGGTTAAAATCATTCG532010.11422782358411102No Hit
CCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTG500050.10736569459828708No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT498180.10696418705124419No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC496900.10668935835594211No Hit
ATTAGGTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGG495770.10644673614837073No Hit
CTGATGGTAAGCTTAAAGTTGAACTGAAATTCTTTTTTGGGCAACCAGCT486580.10447355200006905No Hit
GTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTT486560.10446925780170496No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTGCACCATTGGGAT467440.10036400416563007No Hit
GGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTA467280.1003296505787173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTCT541450.029.1830483
GAGTCTT543150.029.0917074
AGTCTTG569300.028.0852855
TGGAGTC577250.027.2234522
GTCTTGG597100.026.6085056
CCGATTT115400.024.65511
CCCGTTT171300.023.9445861
TCTTGGA690550.023.6850437
CTGGAGT943600.023.5557581
CGATGCG79000.023.0645943
CTTGGAA713050.022.4442588
TTGGAAG712600.022.3978659
CCCGCTA50550.022.2853681
TCGTTTG587300.022.2203352
CGCTATG100850.022.1974031
CCCGTAT50800.022.1756951
CGCTGTT590550.021.8761061
CGTTTGG594200.021.4411243
CGATTTG98500.021.2038212
CCCGAAT76050.021.1749321
Produced by FastQC (version 0.11.3)