FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI04.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI04.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46574467
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC1199600.25756601787842254No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA976900.2097501190942239No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT874520.1877681176684212No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC851920.18291567351699378No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA768910.16509260320681715No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG635990.1365533608790413No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT587030.12604116328373655No Hit
CATAACTAAACCCCCACATAAACTAAAACATTTAACTCAAAAAGTATTGG555100.11918547559545878No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT542750.11653380810563008No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG523620.11242640737037313No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTA503360.10807638442754482No Hit
TGACAACCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCA492110.10566089784774134No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCC478880.10282028562989246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC392250.063.76751
GTGGCGC378350.050.283373
GGTGGCG493100.045.7639472
TACAACC440400.045.3561972
TGGCGCA337250.043.7766534
CTACAAC494950.041.6257251
GCGCACG375650.039.6811456
GGCGCAC414450.036.0452885
CGCACGC465600.032.609991
ACAACCA650350.031.0903433
GCGCCCG143100.030.646086
TGCGACA138750.029.8941886
GCGACAT142700.029.621867
GCACGCC516650.029.0210952
ACCAACC720100.027.7702856
TGGCGCC176450.024.8849664
GGCGCCC179250.024.6610625
CACGCCT619800.024.2824739
GCGGACG84450.024.2167556
CCAACCA851150.023.3000057
Produced by FastQC (version 0.11.3)