FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI05.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI05.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28917858
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT710140.24557143893576072No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG547690.18939507898544905No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT546330.18892478135828733No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT460190.15913695959085214No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA447830.15486278409694107No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA447500.1547486677609386No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG442100.15288130953544346No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT420180.14530121836824844No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG414380.14329553731123518No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA413630.1430361820021386No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG391980.13554945874621832No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT389400.13465727648292622No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT381050.13176978737498468No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT371140.12834283922412232No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG369510.12777917368568584No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT353610.1222808411328391No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA351050.12139557501112289No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG330420.1142615749755739No Hit
ATTAGGTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGG325470.11254982993553672No Hit
CTCCTTTGTCCTTTAGTTTCATAAAGGGTTTCGTAATGTTCTCTGGGAAA315680.10916437863412981No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC300390.10387698839934825No Hit
CTGATGGTAAGCTTAAAGTTGAACTGAAATTCTTTTTTGGGCAACCAGCT295380.10214449493458333No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA290510.10046041446084976No Hit
ATAAGATATTAGTATTACTTTGACTTGTGAGTCTAGGTTAAAATCATTCG290400.10042237568218228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTT72750.025.010571
GAGTCTT315300.024.5064544
GGAGTCT313200.024.4638423
AGTCTTG324850.024.2331685
TGGAGTC332850.022.9546992
GTCTTGG344700.022.7120256
TCGTTTG337300.022.3102662
CGATTTG61300.021.6153432
CCCGATT63900.021.5818461
CTGGAGT538900.021.5309771
CGTTTGG346350.021.3322643
CGTTGAT401800.021.2763861
CGCGTTT18800.020.7391991
CCCGAAT47350.020.5859011
CGATGCG41350.020.3758223
CGCTATG56750.020.1023731
CGCTGTT342950.020.0429761
TCTTGGA405250.019.7269177
CGCGGGT50650.019.387221
CCCGTTT120250.019.214141
Produced by FastQC (version 0.11.3)